Notice: Due to an ongoing redesign most of the articles in the lab section have been pulled to draft until I can rewrite and reformat them. An article framework has been left in place, so if there is anything in particular you’re looking for please feel free to contact me.

Setting up a Yeast Lab

Setting up a basic home microbiology lab

Setting up an intermediate lab

Selecting a Microscope

Purchasing general lab media and supplies

Basic Lab Practices

Media preparation

Aseptic Technique

Autoclaving & Pressure Cookers

Using a Hemocytometer For Cell Counting

Gram Staining: The cornerstone cell staining technique for microbiologists everywhere

Serial Dilution

Streak Plating for Isolation

Yeast Viability Staining

Microbe Handling & Management

Yeast Washing

How to Isolate a Pure Culture

Banking Pure Cultures

Short Term: Streak Plates

Mid Term: Slant Storage

Long Term: Isotonic Storage

Near Indefinite: Cryogenic Storage

Propagating an Archived Sample

Lab Media Recipes

Dekkara/Brettanomyces Differential Media

A complex isolation media formulated to be selective against prokaryotes and non-Brettanomyces/Dekkara yeast, as well as provide aromatic differentiation between Brettanomyces and any non-Brettanomyces yeast that manage to grow.

Rogosa SL Media

A selective media for the growth of facultative anaerobe Lactobacillus, or lactic acid bacteria.

Debaryomyces Differential Medium (DDM)

A selective and differential solid medium used for the easy identification of the extreme osmotolerant yeast species, Debaryomyces hansenii.

YPD Media

MYPG Media